• 1. A type of whole genome sequencing called Sanger Sequencing was used to sequence the first human genome.
• Your Colleagues Responded
• A. True

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• B. False

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• 2. _____ coverage depth results in increased confidence in variant identification.
• Your Colleagues Responded
• A. Decreased

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• B. Increased

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• C. Equal

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• 3. Whole genome sequencing methods include:
• Your Colleagues Responded
• A. Clone-by-clone

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• B. Whole genome shotgun

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• C. Both A and B

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• D. Neither

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• 4. De novo assembly is performed by identifying overlapping regions in the DNA sequences, aligning, the sequences and putting them back together to form the genome. De novo assembly is the only way to sequence a new organism.
• Your Colleagues Responded
• A. True

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• B. False

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• 5. Exomes make up approximately _____% of the human genome.
• Your Colleagues Responded
• A. 2

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• B. 12

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• C. 20

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• D. 90

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• 6. Whole genome sequencing is preferred for use in the _____ setting, while targeted panels are preferred for use in the _____ setting.
• Your Colleagues Responded
• A. Clinical, research

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• B. Research, clinical

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• 7. Strengths of whole genome sequencing using next generation sequencing methods include:
• Your Colleagues Responded
• A. Ability to obtain information on the entire genome

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• B. Detection of DNA abnormalities using less tissue compared to traditional methods

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• C. Less costly than traditional methods

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• D. Faster turnaround time compared to traditional methods

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• E. All of the above

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• 8. For many abnormalities detected by whole genome sequencing, clinical utility is unknown.
• Your Colleagues Responded
• A. True

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• B. False

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• 9. Bioinformaticists are an important component to analyzing whole genome sequencing data. Their workflow includes:
• Your Colleagues Responded
• A. Quality control and data grooming, genome assembly and/or variant calling, and post-assembly analysis

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• B. Genome assembly and/or variant calling, quality control and data grooming, and post-assembly analysis

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• C. Genome assembly and/or variant calling, post-assembly analysis, and quality control and data grooming

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