• 1. Which of the following assays would be considered a form of liquid biopsy?
• Your Colleagues Responded
• A. Quantification of cell-free circulating DNA (cfDNA) in patient with lung cancer

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• B. EGFR mutation testing in circulating tumor DNA (ctDNA) in patient with lung cancer

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• C. Enumeration of circulating tumor cells (CTCs) in peripheral blood from patient with breast cancer

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• D. All of the above

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• 2. Cold ischemic time for tissue specimens being sent to the pathology lab is defined as:
• Your Colleagues Responded
• A. The duration of time the specimen is in refrigeration

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• B. The duration of time between when a specimen is removed from a patient to when it is in contact with formalin

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• C. The duration of time a specimen is in contact with formalin

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• D. The duration of time a specimen is decalcified

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• 3. An example of a predictive biomarker is:
• Your Colleagues Responded
• A. . CA19-9 level in pancreatic cancer

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• B. Expression of CD44 in bladder cancer

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• C. Detection of BRAF V600E mutation in melanoma

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• D. Expression of cyclin D1 in bladder cancer

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• 4. Which of the following pre-analytical factors may affect the antigenicity of tumor tissue used for immunohistochemistry testing?
• Your Colleagues Responded
• A. Decalcification of the specimen

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• B. Under fixation of tissue prior to evaluation

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• C. Prolonged cold ischemic time

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• D. All of the above

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• 5. Which of the following sequencing techniques employs a process similar to capillary electrophoresis?
• Your Colleagues Responded
• A. Sanger sequencing

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• B. Pyrosequencing

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• C. Next-generation sequencing

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• D. None of the above

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