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Certain species dominate oral microbiome in patients with Sjögren’s syndrome
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Certain species of organisms were predominant and others were lacking in abundance in the oral microbiome of patients with primary Sjögren’s syndrome compared with organisms found in the mouths of healthy individuals, according to research from the European League Against Rheumatism Annual European Congress of Rheumatology.
An analysis of organisms present in saliva of 13 patients with primary Sjögren’s syndrome (pSS) and 12 healthy participants was conducted. None of the participants had oral disease or comorbidities, and none had recent use of antibiotics. Exclusion criteria included chronic use of alcohol or tobacco products.
A Qiagen DNA isolation kit was used to extract DNA from the saliva collected in sterile tubes with a buffer solution. Illumina Hiseq 2500 was used to prepare and sequence the libraries, and core sequences were mapped to the Human Oral Microbiome Database. Results were considered significant in the presence of a fold change of greater than two and P values above 0.05 by Student’s t-test.
Organisms found in abundance included Capnocytophaga, Dialister, Fusobacterium, Helicobacter, Streptococcus and Veilonella. Compared with healthy participants, a lack of Pseudomonas bacteria was noted.
According to the researchers, Capnocytophaga has previously been associated with pathogenesis and T cell activation in pSS. – by Shirley Pulawski
Reference:
Sandhya P, et al. Paper #AB0188. Presented at: European League Against Rheumatism Annual European Congress of Rheumatology; June 10-13, 2015; Rome.
Disclosure: The researchers report no relevant financial disclosures.