This article is more than 5 years old. Information may no longer be current.
Proteins could be potential biomarkers in RA, PsA patients
Click Here to Manage Email Alerts
Differences existed in protein expression patterns between inflammatory and noninflammatory rheumatic conditions, with four proteins displaying potential as biomarkers for rheumatoid arthritis and psoriatic arthritis, according to recent research results.
Researchers in Croatia studied plasma samples from 20 patients each with rheumatoid arthritis (RA; aged 21-62 years), psoriatic arthritis (PsA; aged 25-65 years) and noninflammatory arthritis (NIA; aged 24-65 years). Sodium dodecyl sulfate polyacrylamide gel electrophoresis and in-gel digestion with trypsin were conducted after enrichment of proteins with heparin affinity. Liquid chromatography coupled to a mass spectrometer was used to separate, measure, concentrate and micro-purify peptides. MaxQuant software generated peak lists from raw spectra.
Statistical analysis of protein-relative expression levels was conducted. By excluding one protein at a time, individual protein impact on whole dataset correlation was conducted.
Maintaining a false discovery rate of 1%, analysis revealed 384 different proteins, 163 that were identified in all three conditions.
RA and PsA were correlated, according to plasma proteomics. Ten proteins showed an impact on the correlation coefficient falling outside of two standard deviations of the mean. In PsA and RA by a similar ratio when compared with NIA, four proteins (complement factor I, complement component C8 beta, glyceraldehyde-3-phosphate deyhydrogenase and inter-alpha-trypsin-inhibitor heavy chain H1) were up-regulated, while two (immunoglobulin heavy chain V-III region BRO and immunoglobulin J chain) were down-regulated.
Compared with NIA, the remaining proteins (Serpin A11, complement factor H-related protein 5, cartilage acidic protein 1 and coagulation factor IX) were down-regulated in PsA and up-regulated in RA.
“In our preliminary study of RA, PsA and NIA, we found some differences in protein expression pattern between inflammatory and noninflammatory rheumatic conditions,” the researchers concluded. “Out of 384 proteins with heparin affinity, four proteins should be further validated as potential diagnostic biomarkers in patients with RA and PsA.”
Disclosure: The researchers report no relevant financial disclosures.
Click Here to Manage Email Alerts