Mobilized PRP may produce a similar quantity of cells as bone marrow aspirate concentrate
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NASHVILLE, Tenn. — Mobilized platelet-rich plasma produced a similar quantity of cells as concentrated bone marrow aspirate, according to a presenter.
Adam W. Anz, MD, and colleagues performed a bone marrow aspirate and blood draw among 10 patients to make standard concentrated bone marrow aspirate and standard PRP. Researchers processed another blood draw with filgrastim, a synthetic hormone that upregulates the production of progenitor cells within bone marrow which can later be harvested, with a point of care blood processing system.
“Total nucleus cells were similar between the two products, the mobilized PRP and the concentrated bone marrow aspirate,” Anz said in his presentation during the Biologic Association Annual Summit at the American Orthopaedic Society for Sports Medicine and Arthroscopy Association of North America Combined Meeting. “We did find more hematopoietic progenitor cells in the mobilized PRP product.”
He added both the concentrated bone marrow aspirate and mobilized PRP products showed some colony growth, although not the growth they had expected.
“When we looked higher magnification at these, we surely did see cells that were dividing, and we also saw markers that were more consistent with monocytes and other white blood cells,” Anz said.
Anz noted they then wanted to see if these cells were comparable to pluripotent cells, and found the monocytes could reproduce in a culture dish. The monocytes were also able to differentiate into chondrocytes, adipocytes and osteocytes, according to Anz.
“Another thing that was surprising, though, we wanted to look and see if we could push them into endoderm and ectoderm lineages, and we could as well,” Anz said. “So, yes, they were acting similar to pluripotent cells.”
Reference:
Anz AW, et al. Arthroscopy. 2020; doi:10.1016/j.arthro.2020.07.005.
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Anz AW, et al. Quantification and qualification of stem cells from blood after mobilization with Filgrastim, and concentration using a PRP system. Presented at: American Orthopaedic Society for Sports Medicine and Arthroscopy Association of North America Combined Meeting; July 7-11, 2021; Nashville, Tennessee.
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