Age, cell concentration may impact connective tissue progenitor prevalence, concentration
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Published results showed younger age and high cell concentration in bone marrow aspirate may positively impact connective tissue progenitor prevalence and concentration.
Nicolas S. Piuzzi, MD, and colleagues aspirated iliac crest bone marrow from 436 patients during elective total hip or knee arthroplasty and recorded patient age, sex, race, BMI, the presence of osteoporosis and cell concentration. Researchers plated bone marrow-derived nucleated cells and performed a colony-forming unit analysis on day 6.
Results showed a significant influence of age and cell concentration on connective tissue progenitor prevalence and concentration. Researchers found the odds of having at least an average connective tissue progenitor prevalence decreased by 1.5% for every 1-year increase in age, while the odds of having at least an average connective tissue progenitor concentration decreased by 1.6%. Researchers also noted the odds of having at least an average connective tissue progenitor prevalence and concentration increased by 2.2% and 7.9%, respectively, for every 1 million cells/mL increase in cell concentration. However, connective tissue progenitor prevalence or concentration were not influenced by sex, race, BMI and the presence of osteoporosis, according to results.
“Bone marrow aspirate (BMA) is one of the most frequently used sources for cellular therapies in orthopedics. To the best of our knowledge, this study is the largest case series to explore the prevalence of stem and progenitor cells in BMA,” Piuzzi, director of research of adult joint reconstruction and associate staff of adult joint reconstruction surgery at Cleveland Clinic, told Healio Orthopedics. “What we found was that the number of stem and progenitor cells decreases as age increases. Contrarywise, we found that for every 1 million cells/mL increase in BMA cell concentration, the number of stem and progenitor cells increased 7.9%. Therefore, as we cannot modify our patient’s age, this highlights the importance of proper BMA techniques to obtain a high-quality yield and composition of cells to optimize the potential success of bone marrow derived cell therapies.”
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