Real-Time Polymerase Chain Reaction

Approximately 18% of patients who recovered from COVID-19 and tested negative for the virus later tested positive, and a small number may still have been infectious, according to a study published in JAMA Internal Medicine.

Researchers have found three microRNAs that may predict respiratory failure and sepsis among patients with community-acquired pneumonia.

SAN FRANCISCO — Researchers developed a highly sensitive quantitative PCR, or qPCR, method for the detection and quantitation of dengue and Zika virus in individual mosquitoes using the portable Hyris bCUBE device, introducing a new tool for mosquito surveillance in areas facing outbreaks, according to data presented at ASM Microbe.

WASHINGTON, D.C. — In-house real-time polymerase chain reaction assays for detection and identification of intestinal protozoa outperformed commercial multiplex PCR kits, according to data presented at ICAAC 2014.

WASHINGTON, D.C. — Four different assays for the detection of norovirus in stool specimens performed comparably, according to data presented at ICAAC 2014.

WASHINGTON, D.C. — A single-step nucleic acid amplification test in combination with a strict stool rejection policy detected more cases of Clostridium difficile compared with a combination of enzyme immunoassays and toxigenic culture, according to data presented at ICAAC 2014.