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Autoantibody PUF60 uniquely present in patients with Sjögren’s syndrome, dermatomyositis
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Researchers of this study found the autoantibody poly(U)-binding-splicing factor 60 kDa presents distinctly in patients with Sjögren’s syndrome and dermatomyositis.
“We have identified [poly(U)-binding-splicing factor 60 kDa] PUF60 as a novel, commonly targeted autoantigen in [Sjögren’s syndrome] SS and [dermatomyositis] DM,” David F. Fiorentino, MD, PhD, from the Department of Dermatology at Stanford University School of Medicine in Redwood City, Calif., and colleagues wrote in their study. “Understanding the basis for its differential serologic and phenotypic associations in these two diseases will allow greater understanding of mechanisms that trigger and propagate autoimmunity.”
Using a human protein array, 2-D gel electrophoresis and liquid chromatography tandem mass spectrometry peptide sequencing, the researchers identified the antibody as PUF60, according to the abstract. Fiorentino and colleagues then evaluated serum to identify anti-PUF60 antibodies in 267 patients with DM, 84 patients with SS, 71 patients with systemic lupus erythematosus (SLE), 45 patients with polymyositis and 45 patients with inclusion body myositis. All patients were tested against 38 patients in a healthy control group.
Investigators found 30% of patients with SS and 8.5% of patients with SLE had anti-PUF60 antibodies compared with 5% of healthy patients, according to the abstract. Anti-PUF60 antibodies were found in 18% of patients with DM, in 8.9% of patients with inclusion body myositis and in 11.1% of patients with polymyositis. Patients with DM showed a significant association between anti-transcription intermediary factor 1 gamma seropositivity, Caucasian race and PUF60. Fiorentino and colleagues noted factors such as rheumatoid factor, hyperglobulinemia and anti-Ro52 antibodies were significantly associated with PUF60 in patients with SS. – by Jeff Craven
Disclosure: The researchers report no relevant financial disclosures.
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