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New test detected resistant bacteria rapidly, accurately

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Evaluation of the Carba NP test found it to be a rapid, inexpensive and accurate means of detecting carbapenem-resistant Enterobacteriaceae, according to data presented at ICAAC 2014.

“Over the past decade, carbapenemase-producing carbapenem-resistant Enterobacteriaceae (CP-CRE) have rapidly spread around the globe and are currently considered an urgent public health threat by the CDC,” Karim Morey, MS, of the Oregon State Public Health Lab, said in a press release. “Timely detection of CP-CRE is critical to patient care and infection control.”

The study evaluated 196 clinical Enterobacteriaceae through a Carba NP test performed using protocol previously published by the Mayo Clinic. Known isolates OXA-48 and OXA-181, which were initially Carba NP negative, were retested using a modified protocol with increased inoculum size in a reduced volume of lysis solution.

The test’s sensitivity and specificity was 92% and 100%, respectively. It detected all tested carbapenemase producers while detecting zero of five OXA-48 and OXA-181 strains. When the modified protocol was applied, all five strains were detected. The test, performed in triplicate with subsets tested by different analysts, was 100% reproducible and took an average of 2.5 hours.

“The Carba NP test is highly sensitive, specific and reproducible for the detection of carbapenemase production in a diverse group of Enterobacteriaceae collected from a region with low carbapenem-resistant Enterobacteriaceae prevalence.” the researchers concluded.

For more information:

Morey K. Abstract D-888. Presented at: 2014 Interscience Conference on Antimicrobial Agents and Chemotherapy; Sept. 5-9; Washington, D.C.

Disclosure: The researchers report no relevant financial disclosures.